Zinc
Application Time: AM / Morning
Zinc is an essential trace mineral that plays a foundational role in cell growth, repair and immune function. It is widely recognized in dermatology and nutrition for its supportive effects on skin health, particularly in maintaining balanced oil production, promoting barrier integrity and assisting with wound healing. Within the body, zinc is deeply involved in processes that regulate cellular ageing. Research from institutions such as the University of California, Berkeley, highlights zinc’s influence on cellular senescence—the natural process by which cells lose their ability to divide and renew over time. By supporting healthy cell development and DNA integrity, zinc contributes to stronger, more functional tissues throughout the body. As zinc levels naturally decline with age, the body’s ability to maintain and repair tissue can diminish. Ensuring adequate zinc intake supports: Healthy skin renewal and barrier strength Balanced oil production and clearer complexion Reduced oxidative stress and inflammation Enhanced immune resilience Natural tissue repair and collagen-supporting pathways Because of its role in maintaining structural and cellular vitality, zinc is often used in oral supplements supporting conditions like acne-prone or sensitive skin, as well as healthy-ageing formulations.
Vitamin D3
Application Time: PM / Evening
Vitamin D3 is a unique, hormone-like nutrient that the body produces primarily through sunlight exposure. Despite abundant sunshine in countries like South Africa and Australia, low Vitamin D levels remain common due to genetic, environmental and lifestyle factors, making dietary and supplemental sources increasingly important. Vitamin D3 plays a vital role in regulating over 200 genes, contributing to healthy cellular function, DNA support, immune balance and whole-body vitality. It is essential for maintaining bone strength, muscle performance, mood regulation and immune system resilience. As a key nutrient involved in cellular protection, Vitamin D3 helps support the body’s natural defenses against oxidative stress and inflammation. By promoting balanced inflammatory responses, Vitamin D3 contributes to joint comfort, cardiovascular wellbeing and healthy-ageing pathways throughout the body. Vitamin D3 also supports: Positive mood and emotional wellbeing Strong bones and muscles Healthy immune responses Overall metabolic and cardiovascular balance Neurological and cognitive health With its broad, system-wide benefits, Vitamin D3 is considered one of the most important nutrients for maintaining long-term wellness, cellular integrity and whole-body vitality.
Vitamin C (Ascorbic Acid)
Application Time: AM & PM / Morning & Evening
Vitamin C is a powerful water-soluble antioxidant that plays a central role in skin health, immune function and cellular protection. Vitamin C complements the antioxidant system by supporting tissue repair, collagen formation and healthy inflammation balance. As a key cofactor in the synthesis of collagen and elastin, Vitamin C is essential for maintaining firm, resilient and youthful-looking skin. Its ability to strengthen the skin’s structural matrix helps support smoother texture, improved elasticity and enhanced overall radiance. Vitamin C also provides important anti-glycation benefits, helping to protect proteins like collagen from oxidative and sugar-related damage. By neutralizing free radicals, Vitamin C helps defend the skin against the visible signs of photoaging and environmental stress. In dermatologic research, Vitamin C has been shown to support: Faster wound and tissue healing Protection against UV-induced oxidative stress Improved skin tone and brightness Reduced appearance of pigmentation through antioxidant pathways By boosting collagen production, encouraging new skin growth, and reinforcing the skin’s natural defense systems, Vitamin C plays a vital role in both skin rejuvenation and overall cellular vitality.
Trimethylglycine (TMG)
Application Time: AM / Morning
Trimethylglycine (TMG), also known as betaine, is a key nutrient involved in one of the body’s most essential liver detoxification pathways: methylation. Methylation supports hundreds of biochemical reactions, including cellular repair, neurotransmitter production, energy metabolism and toxin processing. As a major methyl donor, TMG helps maintain efficient detoxification by supporting the conversion of homocysteine into methionine — a critical step for balanced metabolic function and reduced oxidative stress. When methylation is supported, the body is better equipped to manage toxic byproducts, maintain optimal cellular health and regulate inflammation. A well-functioning methylation cycle is essential for: Healthy liver detoxification Effective antioxidant defense Balanced inflammation response Stable energy metabolism Cellular protection and repair Without adequate methylation support, toxin load and oxidative stress can accumulate, contributing to chronic cellular strain. TMG helps maintain this pathway’s efficiency, promoting a clearer internal environment and supporting overall vitality. As part of the NADplus+ Complex, Trimethylglycine strengthens the body’s foundational detox and metabolic systems, aiding in healthy ageing, cellular resilience and whole-body wellbeing.
Sulforaphane (Broccoli Extract)
Application Time: AM / Morning
Sulforaphane is a potent isothiocyanate naturally derived from broccoli and other cruciferous vegetables. It is widely recognized for its ability to activate the body’s own detoxification, antioxidant and protective pathways, making it one of the most researched plant-based compounds in longevity and cellular health science. One of sulforaphane’s key strengths is its ability to boost Phase II detoxification enzymes, helping the body process and neutralize environmental toxins and reactive compounds. This supports the liver’s natural cleansing systems and contributes to enhanced metabolic balance and cellular resilience. In skin and cellular research, sulforaphane has been shown to help support DNA integrity, offering protection against oxidative stressors such as reactive oxygen species (ROS), pollutants and other environmental challenges. Its anti-inflammatory properties further reinforce healthy cellular function and contribute to a more balanced, youthful biological environment. Sulforaphane is also known to upregulate metallothionein gene expression, aiding the body in managing certain heavy metals and supporting internal detoxification mechanisms. Together, these actions position sulforaphane as a powerful ingredient for promoting: Cellular protection and repair Healthy detoxification pathways Balanced inflammation response Immune system support Holistic healthy-ageing benefits Within the NADplus+ Complex, sulforaphane works synergistically with other antioxidants and detoxification nutrients to enhance cellular vitality, resilience and long-term wellbeing
Selenium
Application Time: AM / Morning
Selenium is an essential trace mineral required for numerous biological processes, particularly those related to cellular protection, antioxidant defense and healthy ageing. It plays a critical role in supporting enzymes that defend against oxidative stress, including glutathione peroxidase (GPx1)—a key antioxidant enzyme that depends on selenium to function. As we age, cells—especially in sensitive areas like the brain—can accumulate harmful byproducts such as methyl mercury, which contributes to cellular stress and decline. Selenium has been shown to help neutralize certain reactive compounds, supporting the body’s natural detoxification mechanisms and helping maintain healthier cellular function over time. Selenium’s biological influence extends to: Protection against oxidative damage Support for DNA synthesis and repair Normal immune and metabolic function Defense against reactive oxygen species (ROS) Research has explored selenium’s potential role in supporting skin health, particularly due to its antioxidant properties and its ability to support cellular resilience against environmental stressors. Certain organic forms of selenium—such as selenium-enriched yeast containing selenomethionine—are recognized for having higher bioavailability and lower toxicity compared to inorganic forms like sodium selenite, making them preferable in advanced nutritional formulations. Within NADplus+ Complex, selenium works synergistically with other antioxidants to strengthen the body’s natural defense systems, promote cellular vitality and support balanced ageing from within.
Royal Jelly (apis melifera)
Application Time: AM / Morning
Royal Jelly is a highly bioactive peptide-rich secretion produced by the hypopharyngeal and mandibular glands of worker honeybees. For the first three days of life, all bee larvae are fed Royal Jelly; however, it is the exclusive lifelong nourishment of the queen bee—a remarkable distinction associated with her substantially longer lifespan, larger size and stronger vitality compared to worker bees. Chemically, Royal Jelly is a complex and nutrient-dense substance composed of proteins, peptides, lipids, sugars, vitamins, minerals and free amino acids, including unique bioactive fractions not found elsewhere in nature. These components give Royal Jelly its long-standing reputation as a premium ingredient in nutritional and cosmetic formulations. In cellular studies, Royal Jelly has shown the ability to reduce intracellular oxidative stress in a dose-dependent manner, contributing to enhanced cellular protection. It has also been observed to influence cell growth, energy metabolism and protein expression, suggesting that its benefits extend beyond antioxidant activity alone. Royal Jelly appears to help modulate the cellular environment by affecting the expression of specific proteins involved in function, repair and resilience. This multifunctional profile positions Royal Jelly as a valued biopeptide in wellness and healthy-ageing formulations. By supporting antioxidant defence, cellular energy and balanced protein activity, Royal Jelly aligns with the core principles of NADplus+ Complex—promoting vitality, cellular balance and whole-body wellbeing.
Resveratrol
Application Time: AM / Morning
Resveratrol is a naturally occurring polyphenol antioxidant found in red grape skins, berries, peanuts and cacao. It is widely studied for its potential role in healthy ageing, cellular protection and antioxidant defense. Its strong scientific interest comes from its ability to influence pathways linked to longevity, inflammation and oxidative stress. Early studies, including a well-known 2006 mouse study, showed that resveratrol could mimic some of the biological effects of calorie restriction—a dietary pattern associated with improved metabolic health and extended lifespan. While research continues, these findings have positioned resveratrol as one of the most compelling natural compounds in the field of longevity science. Resveratrol provides potent antioxidant support, helping to neutralize free radicals and reduce oxidative stress throughout the body. Because oxidative stress is a key driver of premature ageing, resveratrol’s protective actions may help support healthier, more resilient cells over time. In dermatology research, resveratrol has shown the ability to help protect skin from UVA-induced damage, the wavelength most responsible for photo-ageing. It has also demonstrated potential to support collagen production and contribute to smoother, firmer, more youthful-looking skin. Additional emerging research suggests that resveratrol may support bone health, assist in balancing changes associated with menopause and modulate enzymes such as tyrosine kinase that are linked to degenerative processes. Although red wine naturally contains resveratrol, the levels are low (approximately 1.5–3 mg per liter). Supplemental resveratrol provides concentrated and consistent levels ideal for wellness formulations.
Niacin- Nicotinic Acid (Vitamin B3)
Application Time: AM / Morning
Niacin, also known as Vitamin B3, is an essential water-soluble vitamin and a key member of the B-vitamin complex. It plays a central role in maintaining healthy cellular function, energy production and skin vitality. As a precursor to NAD⁺ and NADP, two coenzymes vital for life-sustaining biochemical reactions, niacin supports oxidation–reduction processes, ATP (energy) synthesis and cellular repair mechanisms. Emerging research highlights niacin’s growing importance in dermatology. In vitro studies suggest that Vitamin B3 may help increase collagen production and reduce hyperpigmentation, contributing to a brighter, smoother and more even-toned complexion. It is also being explored for its potential benefits in supporting common skin concerns such as acne and photo-ageing. By promoting efficient energy transfer within cells, niacin helps ensure that cellular processes function optimally. Healthy, well-energized cells are more resilient, perform better and maintain their structural integrity throughout their normal lifespan. As part of the NADplus+ Complex, niacin supports: Healthy NAD⁺ production Efficient cellular energy metabolism Enhanced antioxidant defense Skin barrier, tone and collagen-support pathways Niacin is essential for maintaining vibrant skin and cellular vitality, making it a foundational nutrient in holistic healthy-ageing formulations.
N-Acetyl Cysteine (NAC)
Application Time: AM / Morning
N-Acetyl Cysteine (NAC) is a biologically active form of the sulfur-containing amino acid cysteine and an essential precursor for the production of glutathione—one of the body’s most important natural antioxidants. By supporting glutathione synthesis, NAC plays a vital role in cellular protection, detoxification pathways and the neutralisation of oxidative stress. Cysteine is deeply involved in the body’s sulfation and methylation cycles, helping convert homocysteine to methionine, supporting healthy detoxification processes and contributing to balanced metabolic function. NAC enhances this process by being rapidly converted into intracellular glutathione, helping the body break down and transform reactive compounds into less harmful byproducts. NAC is widely recognized for supporting the body’s natural detoxification pathways, particularly in assisting with the clearance of certain environmental toxins. Its antioxidant properties also help defend cells from free-radical damage, supporting DNA integrity and promoting optimal cellular repair. Within the NADplus+ Complex, NAC strengthens the body’s resilience by: Supporting glutathione production Enhancing natural detoxification processes Protecting cells against oxidative stress Contributing to healthy cellular function and longevity NAC's broad systemic benefits make it a key component in formulations designed to promote cellular vitality, metabolic balance and holistic wellbeing.
Magnesium
Application Time: AM / Morning
Magnesium is one of the most essential minerals in human health, required for more than 300 biochemical reactions in the body. It supports vital physiological functions — from muscle and nerve activity to energy production, cardiovascular balance and a strong, healthy immune system. Recognised by both the NIH and EFSA, magnesium contributes to: Normal muscle and nerve function Steady heart rhythm and cardiovascular support Healthy immune response Bone and teeth strength Protein synthesis and cellular repair Reduced tiredness and fatigue Energy-yielding metabolism Magnesium also plays a key role in glucose regulation. It supports glucose oxidation and helps transport glucose across cell membranes — processes essential for stable energy levels and metabolic health. Research shows that low magnesium status may be linked to impaired glucose control and a higher risk of metabolic disorders. Beyond metabolic benefits, magnesium is increasingly recognised for its importance in neurotransmission, cognitive function and muscle relaxation, making it invaluable for modern lifestyles affected by stress, poor sleep or high physical demand. Magnesium contributes to overall cellular vitality, metabolic balance and healthy-ageing support, reinforcing the body’s natural systems for sustained wellness and resilience.
L-Proline (Amino Acid)
Application Time: AM / Morning
L-Proline is a key collagen-building amino acid and an essential component of the NADplus+ Complex. As one of the primary structural elements of collagen and elastin, L-Proline plays a crucial role in maintaining the strength, stability and resilience of the skin, joints, ligaments and other connective tissues. Because L-Proline supports collagen’s triple-helix structure, it helps protect against accelerated ageing in both skin and cartilage. When combined with Vitamin C — a co-factor in collagen synthesis — its effectiveness is further enhanced, making this pairing especially valuable for supporting firmness, elasticity and smoothness in the skin. Beyond its structural importance, L-Proline is also used for supporting energy, reducing stress and fatigue, and promoting a general sense of vitality. As part of NADplus+ Complex, it contributes to a more holistic approach to healthy ageing, reinforcing the body’s natural ability to maintain strong connective tissues and radiant, youthful-looking skin.
L-Carnosine
Application Time: AM / Morning
L-Carnosine is a naturally occurring dipeptide (a combination of two amino acids: beta-alanine and histidine) found in high concentrations in muscle and nerve tissue. Levels of L-Carnosine naturally decline with age and this decrease is closely associated with reduced cellular vitality and visible signs of ageing. Widely recognized in longevity science, L-Carnosine is valued for its exceptional antioxidant and anti-glycation properties. Glycation is a process where sugars bind to proteins like collagen, creating stiff, dysfunctional structures that contribute to wrinkles, loss of elasticity and weakened skin support. L-Carnosine helps protect collagen from this cross-linking process, supporting smoother, firmer, more resilient skin. In addition to defending against oxidative and glycation-related damage, L-Carnosine helps maintain the structure of the extracellular matrix, supports cellular health and contributes to balanced tissue renewal. Research also shows that it can help delay cellular senescence, supporting healthier-looking skin over time. Because its benefits extend far beyond the skin — influencing muscle function, connective tissue resilience and cellular protection — L-Carnosine is considered a key ingredient in holistic healthy-ageing formulas.
Glycine (Amino Acid)
Application Time: AM / Morning
Glycine is one of the most abundant amino acids in the human body and a key building block of collagen, making up nearly one-third of its structure. As a major component of the skin, muscles and connective tissues, glycine is essential for maintaining firmness, elasticity and structural integrity. In the skin, glycine supports the body’s ability to repair damaged tissue, helping maintain resilience against UV stress, oxidative damage and daily environmental exposure. Its regenerative properties contribute to smoother, stronger skin and play a vital role in wound healing and tissue renewal. Because glycine is fundamental to collagen production, adequate levels help prevent the skin from becoming slack or weakened over time. It also supports overall connective tissue health, ensuring flexibility, firmness and long-term structural support. Glycine’s presence in collagen-rich supplements helps reinforce the body’s natural repair processes, promoting youthful skin, healthy joints and stronger connective tissue from within.
Coenzyme Q10 (CoQ10)
Application Time: AM / Morning
Coenzyme Q10 is a powerful, naturally occurring antioxidant and cellular energizer essential for maintaining youthful, healthy cells. It plays a key role in mitochondrial energy production, supporting vitality, resilience and optimal cellular repair. One of the most important contributions of CoQ10 is its ability to help protect the body from the damaging effects of Advanced Glycation End Products (AGEs). Glycation is a natural chemical process where sugars bind to proteins, causing them to stiffen, weaken and lose their normal function. This same “browning” reaction that occurs in toasted bread or roasted foods can also occur within the body — contributing to loss of elasticity, increased oxidative stress and accelerated signs of ageing. AGE-modified proteins can generate up to 50 times more free radicals than normal proteins, amplifying oxidative stress and contributing to visible skin ageing, inflammation and reduced cellular performance. CoQ10 helps counteract this process by providing potent antioxidant defense and supporting the body’s natural ability to reduce oxidative stress. When combined with antioxidants such as Resveratrol, CoQ10 forms a powerful shield against glycation-related damage, supporting: Improved cellular resilience Enhanced energy production Protection against oxidative stress Support for healthy ageing and athletic performance Together, these benefits make CoQ10 an essential component in the NADplus+ Complex, helping to protect collagen, preserve skin firmness and maintain overall cellular vitality.
Biotin (Vitamin B7)
Application Time: AM / Morning
Biotin, also known as Vitamin B7, is a water-soluble vitamin and essential coenzyme involved in numerous metabolic processes. It plays a key role in lipid, protein and carbohydrate metabolism, helping the body convert food into usable energy and supporting overall cellular function and vitality. Biotin is especially important for the health of hair, skin and nails. Adequate levels support strong keratin infrastructure, which helps maintain hair thickness, nail strength and healthy skin barrier function. Deficiency in Biotin may lead to hair thinning or shedding (alopecia), brittle or weak nails and red, scaly skin rashes, particularly on the face. Beyond structural benefits, Biotin also contributes to nervous system function. Low levels can result in neurological symptoms such as lethargy, low mood, numbness, tingling or cognitive sluggishness. As part of the NADplus+ Complex, Biotin supports energy metabolism, cellular health and beauty from within, reinforcing healthier-looking skin, stronger nails and more resilient hair.
Astaxanthin
Application Time: AM / Morning
Astaxanthin is one of the most powerful natural antioxidants known, naturally derived from marine algae and responsible for the vibrant pink-red hue of salmon and flamingos. Studies have shown that Astaxanthin can be up to 60 times more potent than well-known antioxidants such as Vitamin E, Vitamin A, lutein and pycnogenol — making it an exceptional ingredient for deep cellular protection. Within the NADplus+ Complex Antioxidant Blend, Astaxanthin helps neutralize free radicals and reduce oxidative stress throughout the body. Its potent anti-inflammatory properties play a central role in supporting skin health, helping to reduce redness, protect against environmental damage and maintain a calm, balanced complexion. Recognized by many leading experts in longevity and anti-ageing science, Astaxanthin has been associated with improvements in wrinkles, age spots, skin moisture, elasticity, smoothness and overall radiance. Its ability to stabilise cell membranes and enhance mitochondrial function makes it a formidable ally in promoting youthful, resilient and vibrant skin. Paired with the detoxification substrates in NADplus+ Complex, Astaxanthin works synergistically to deliver advanced antioxidant defence, supporting overall wellbeing and contributing to a healthier, more luminous appearance from within.
Alpha Lipoic Acid (ALA)
Application Time: AM / Morning
Alpha Lipoic Acid is often called the “Universal Antioxidant” because it is both water- and fat-soluble, allowing it to work in virtually every cell of the body. As a powerful antioxidant, ALA helps protect skin and tissues from oxidative stress, supporting healthier, more resilient cells and contributing to visible anti-ageing benefits. Beyond its antioxidant strength, Alpha Lipoic Acid may help reduce inflammation, supporting calmer, more balanced skin. Early studies also suggest that ALA may play a role in detoxification pathways, aiding the body in defending itself against environmental and metabolic stressors that can impact overall skin appearance. Research is ongoing, but ALA has shown potential in influencing blood sugar regulation. This is particularly relevant for individuals whose skin conditions—such as acne, rosacea or inflammation-driven concerns—may be aggravated by fluctuating glucose levels. By helping to stabilize these processes, Alpha Lipoic Acid may support clearer, calmer and more even-toned skin.
NAD⁺ (Nicotinamide Adenine Dinucleotide)
Application Time: AM / Morning
NAD⁺ is a naturally occurring coenzyme found in every cell, essential for life-sustaining energy production. It plays a central role in the mitochondria – the “power plants” of our cells – where it helps convert nutrients into ATP, the energy currency the body relies on for optimal function, repair and resilience. As a key oxidation–reduction (redox) cofactor, NAD⁺ accepts and donates electrons during metabolic reactions, helping to regulate cellular metabolism and support healthy tissue function. Adequate NAD⁺ levels are associated with better cellular energy, improved performance and enhanced stress tolerance at a cellular level. NAD⁺ is also an essential partner for sirtuins – a family of proteins involved in DNA maintenance, cell metabolism, stress responses and healthy ageing pathways. By acting as a substrate for these enzymes, NAD⁺ supports mechanisms linked to cellular repair, genomic stability and longevity support. Emerging research suggests that NAD⁺ helps protect telomeres, the protective caps at the ends of chromosomes and may play a role in maintaining cellular youthfulness over time. In this way, NAD⁺ is increasingly recognised as a powerful ally in supporting healthy ageing from within, contributing to sustained energy, vitality and overall cellular wellbeing.
Sodium Pyruvate
Application Time: AM / Morning
Sodium Pyruvate is a bioactive compound derived from pyruvic acid, central to the body’s energy production pathways (glycolysis and the citric acid cycle). By supporting cellular energy metabolism, it helps protect cells from damage caused by environmental and oxidative stress, making it a valuable ally in healthy ageing and vitality. With anti-inflammatory, antioxidant and anti-ageing properties, Sodium Pyruvate contributes to skin health by reducing inflammation, defending against environmental aggressors and gently promoting the shedding of dead surface cells for a fresher, more radiant complexion. Beyond the skin, Sodium Pyruvate supports joint health by helping to shield cartilage from degradation, aiding in the maintenance of mobility and flexibility. Its protective effects on mitochondria extend to cognitive health, where it is associated with enhanced mental clarity and performance. As part of the COLLAGENplus+ Complex, Sodium Pyruvate offers a holistic approach to wellness, supporting NAD⁺ production and promoting youthful energy, resilience and overall well-being.